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bme  (R&D Systems)


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    Structured Review

    R&D Systems bme
    Bme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stem+cell+factor/Cultrex+ReadyBME%2C+Stem+Cell+Qualified%2C+Reduced+Growth+Factor/pm42031998-58-6-7
    Average 93 stars, based on 6 article reviews
    bme - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Removal of promoter CpG methylation by epigenome editing reverses HBG silencing.
    Article Snippet: .. Human umbilical cord blood derived 2 (HUDEP2) cells were grown in serum free expansion medium (SFEM, StemSpan #9650) supplemented with 50 ng/mL stem cell factor (R&D Systems, #255-SC-200/ CF), 3 IU/mL erythropoietin (PeproTech, #100-64-100), 10 μM dexamethasone (Sigma-Aldrich, #D4902), 1 μg/mL doxycycline (SigmaAldrich, #D9891), and 0.5mg/mL PSG (Thermo Fisher Scientific, #10378016). ..

    Article Title: Removal of promoter CpG methylation by epigenome editing reverses HBG silencing
    Article Snippet: .. Human umbilical cord blood derived 2 (HUDEP2) cells were grown in serum free expansion medium (SFEM, StemSpan #9650) supplemented with 50 ng/mL stem cell factor (R&D Systems, #255-SC-200/CF), 3 IU/mL erythropoietin (PeproTech, #100-64-100), 10 μM dexamethasone (Sigma-Aldrich, #D4902), 1 μg/mL doxycycline (Sigma-Aldrich, #D9891), and 0.5 mg/mL PSG (Thermo Fisher Scientific, #10378016). ..

    Incubation:

    Article Title: Expression and treatment of ROR1 + cells with bispecific T-cell engagers in pediatric acute lymphoblastic leukemia
    Article Snippet: ROR1 density was measured on ROR1 + cells, using a fluorescence quantification kit (Quantibrite; BD Biosciences) and depicted as number of ROR1 binding sites. .. To investigate proliferative capacity, ALL cells were incubated with CellTrace violet (CTV; ThermoFisher Scientific, Altrincham, United Kingdom), according to manufacturer’s instructions, and seeded at 5 × 10 5 cells per mL in Iscoves modified Dulbecco’s medium with Glutamax (Invitrogen, Paisley, United Kingdom) supplemented with rhIL-3 (recombinant human interleukin-3; 20 ng/mL), rhIL-7 (20 ng/mL), stem cell factor (50 ng/mL; all from R&D Systems, Abingdon, United Kingdom), and human insulin transferrin serum substitute (20% volume-to-volume ratio, human serum albumin, insulin, and transferrin; StemCell Technologies, Cambridge, United Kingdom). ..

    Modification:

    Article Title: Expression and treatment of ROR1 + cells with bispecific T-cell engagers in pediatric acute lymphoblastic leukemia
    Article Snippet: ROR1 density was measured on ROR1 + cells, using a fluorescence quantification kit (Quantibrite; BD Biosciences) and depicted as number of ROR1 binding sites. .. To investigate proliferative capacity, ALL cells were incubated with CellTrace violet (CTV; ThermoFisher Scientific, Altrincham, United Kingdom), according to manufacturer’s instructions, and seeded at 5 × 10 5 cells per mL in Iscoves modified Dulbecco’s medium with Glutamax (Invitrogen, Paisley, United Kingdom) supplemented with rhIL-3 (recombinant human interleukin-3; 20 ng/mL), rhIL-7 (20 ng/mL), stem cell factor (50 ng/mL; all from R&D Systems, Abingdon, United Kingdom), and human insulin transferrin serum substitute (20% volume-to-volume ratio, human serum albumin, insulin, and transferrin; StemCell Technologies, Cambridge, United Kingdom). ..

    Article Title: Toggling of NKG2A expression drives functional specialization of iPSC-derived CAR NK cells
    Article Snippet: .. Human iPSCs were differentiated into hematopoietic progenitors and then into CD34+ cells as previously described., , , Upon the appearance of CD34+ cells, CD34+ hematopoietic progenitors were transferred into NK cell differentiation B0 medium containing a 2:1 mixture of Dulbecco modified Eagle medium/Ham F12 (Thermo Fisher Scientific, Waltham, MA, 11965092, 11765054), 2mM L-glutamine (Thermo Fisher Scientific, Waltham, MA, 25030081), 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, 25030081), 25uM b-mercaptoethanol (Gibco, M3148), 10% heat-inactivated human serum AB (Sigma, H3667-100M), 5ng/ml sodium selenite (Merck Millipore, Burlington, MA, S5261), 50uM ethanolamine (Sigma-Aldrich 02400), 20mg/ml ascorbic acid (MerckMillipore, Burlington, MA, A4544), interleukin-3 (IL-3; R&D Systems Minneapolis, MN, 203-IL); for first week only), stem cell factor (SCF; R&D Systems Minneapolis, MN, 7466-SC), interleukin-15 (IL-15; R&D Systems Minneapolis, MN, 247-ILB), Fml-like tyrosine kinase 3 ligand (FLT3L; R&D Systems Minneapolis, MN, 207-IL). ..

    Recombinant:

    Article Title: Expression and treatment of ROR1 + cells with bispecific T-cell engagers in pediatric acute lymphoblastic leukemia
    Article Snippet: ROR1 density was measured on ROR1 + cells, using a fluorescence quantification kit (Quantibrite; BD Biosciences) and depicted as number of ROR1 binding sites. .. To investigate proliferative capacity, ALL cells were incubated with CellTrace violet (CTV; ThermoFisher Scientific, Altrincham, United Kingdom), according to manufacturer’s instructions, and seeded at 5 × 10 5 cells per mL in Iscoves modified Dulbecco’s medium with Glutamax (Invitrogen, Paisley, United Kingdom) supplemented with rhIL-3 (recombinant human interleukin-3; 20 ng/mL), rhIL-7 (20 ng/mL), stem cell factor (50 ng/mL; all from R&D Systems, Abingdon, United Kingdom), and human insulin transferrin serum substitute (20% volume-to-volume ratio, human serum albumin, insulin, and transferrin; StemCell Technologies, Cambridge, United Kingdom). ..

    Stem Cell:

    Article Title: Human iPSC-Derived Mononuclear Phagocytes Improve Cognition and Neural Health across Multiple Mouse Models of Aging and Alzheimer's Disease.
    Article Snippet: Once each dish had roughly 60–80 colonies measuring 1.0 mm in diameter on average, differentiation was initiated using stage 1 media, consisting of 80 ng/ml bone morphogenetic protein 4 (BMP4; #314-BP-010, R&D Systems) in mTeSR. .. Media was changed daily for 4 days, at which point cells were switched to stage 2 media, consisting of 25 ng/ml basal fibroblast growth factor (bFGF; #01-106, Millipore Sigma), 100 ng/mL stem cell factor (SCF; #255-SC-010, R&D Systems) and 80 ng/mL vascular endothelial growth factor (VEGF; #293-VE010, R&D Systems) in StemPro-34 serum free medium (#10 639 011, Gibco). .. Two days later, cells were switched to stage 3 media, consisting of StemPro-34 supplemented with 50 ng/ml SCF, 50 ng/ml interleukin-3 (IL-3; #203-IL-010, R&D Systems), 5 ng/ml thrombopoietin (TPO; #288- TP-005, R&D Systems), 50 ng/mL macrophage colony stimulating factor (M-CSF; #216-MC-010, R&D Systems), and 50 ng/mL fms-related tyrosine kinase 3 ligand (Flt3; #308-FK-005, R&D Systems).

    Cell Differentiation:

    Article Title: Toggling of NKG2A expression drives functional specialization of iPSC-derived CAR NK cells
    Article Snippet: .. Human iPSCs were differentiated into hematopoietic progenitors and then into CD34+ cells as previously described., , , Upon the appearance of CD34+ cells, CD34+ hematopoietic progenitors were transferred into NK cell differentiation B0 medium containing a 2:1 mixture of Dulbecco modified Eagle medium/Ham F12 (Thermo Fisher Scientific, Waltham, MA, 11965092, 11765054), 2mM L-glutamine (Thermo Fisher Scientific, Waltham, MA, 25030081), 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, 25030081), 25uM b-mercaptoethanol (Gibco, M3148), 10% heat-inactivated human serum AB (Sigma, H3667-100M), 5ng/ml sodium selenite (Merck Millipore, Burlington, MA, S5261), 50uM ethanolamine (Sigma-Aldrich 02400), 20mg/ml ascorbic acid (MerckMillipore, Burlington, MA, A4544), interleukin-3 (IL-3; R&D Systems Minneapolis, MN, 203-IL); for first week only), stem cell factor (SCF; R&D Systems Minneapolis, MN, 7466-SC), interleukin-15 (IL-15; R&D Systems Minneapolis, MN, 247-ILB), Fml-like tyrosine kinase 3 ligand (FLT3L; R&D Systems Minneapolis, MN, 207-IL). ..



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